Indentification of cancer stem-like cells in osteosarcoma and their implications in response to chemotherapy
نویسندگان
چکیده
Methods CSCs were isolated using the sphere-forming assay in serum-free medium under non-adherent conditions and characterized for the expression of mesenchymal stem cell markers (CD90, CD105, CD73) by flow cytometry. The chemosensitity of MNNG and CSCs to doxorubicin (DOX), cisplatin (CIS) and methotrexate (MTX) was evaluated using the MTT colorimetric assay after an incubation period of 48h. Cell cycle progression was analyzed by flow cytometry with propidiun iodide.
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